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Fluorescent In Situ Hybridization Fish - USA

price: contact company for price

Fluorescence in situ hybridization (FISH) techniques allow specific

nucleic acid sequences to be detected in morphologically preserved

chromosomes, cells or tissue sections. In combination with

immunocytochemistry, Fluorescence in situ hybridization can relate

microscopic topological information to gene activity at the DNA, mRNA,

and protein level. FISH has become a molecular diagnostic technique

utilizing fluorescently labeled DNA probes to detect or confirm gene or

chromosome abnormalities. The sample DNA (metaphase chromosomes or

interphase nuclei) is first denatured, a fluorescently labeled probe of

interest is then added to the denatured sample mixture and hybridizes

with the sample DNA at the target site as it re-anneals back into a

double helix. The probe signal can then be seen through a fluorescent

microscope and the sample DNA can be scored for the presence or absence

of the signal. Unlike most other techniques used to study chromosomes,

FISH does not have to be performed on cells that are actively dividing.

This makes it a very versatile procedure. This technique is useful for

identifying chromosomal abnormalities, gene mapping, characterizing

somatic cell hybrids, identifying amplified genes and studying the

mechanism of rearrangements. RNA ISH is used to measure and localize

mRNAs and other transcripts within tissue sections or whole mounts.

Company Contact:

  • Phone: 16316244882

  • Address: 45-16 Ramsey Road Shirley, New York, NY, USA

  • Email: Email

Published date: May 6, 2013 -

  • Business Description: involved in international trade import export of goods, products, and services

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