In Situ Hybridization Ish - USAprice: contact company for price
(ISH) service from probe design, tissue acquisition to expert
interpretation of gene expression results.
Our well-established standard operating procedures allow efficient optimization of the staining conditions of a novel probe. Also, we have extensive staining experience in dealing with mouse and human tissue slides or arrays of distinct types. Due to the inherent complexity of this technique, appropriate labeling and hybridization controls are always included in each project. In particular, control housekeeping gene probes are employed to determine the mRNA quality and abundance in tissues provided by customers. Frequently, we use control tissues to validate the results.
A. Template Preparation, e.g. GenBank Accession Number, Gene ID, MGC clone...
B. Probe Design, Labeling and Purification, e.g. radio-, fluorescent- or antigen-labeling
C. Preparation of Tissue
Mouse tissue slides
Human tissue slides
Customer provided tissues.
D. Pilot Hybridization - optimization of ISH hybridization conditions
E. Hybridization - both a sense and an anti-sense probe are used
F. Result Interpretation in a final report
- Posted By: Creative Biogene
- Phone: 16316244882
- Address: 45-16 Ramsey Road Shirley, New York, NY, USA
Published date: May 6, 2013 -
- Business Description: involved in international trade import export of goods, products, and services
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