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Recombinant Enterokinase - China

price: contact company for price

Description Recombinant Enterokinase is a highly

purified preparation of the light chain of bovine enterokinase with

high specificity and recognizes the identical cleavage site as the

native enzyme. The product cleaves fusion proteins with sequence (Asp-Asp-Asp-Asp-

Lys ) at the C-terminal of Lysine. The enzyme is produced in

recombinant E.coli and purified to high homogeneity with specificity

of 30, 000U/mg. It can work at various different conditions, such as

4-45 degree, pH4.5-9.5, even in some detergents or denature reagents.

Activity One unit is defined as the amount of enzyme that will

cleave 95% of 50μg fusion protein in 16 hours at 23℃ in 1x

reaction buffer. 10x Reaction Buffer 500 mM Tris-HCl,

pH 8.0 10 mM CaCl 2 1% Tween-20 (v/v)

1. For 1 liter, dissolve 60.5 g Tris base in 950 ml

deionized water. 2. Adjust pH to 8.0 with concentrated

HCl. 3. Add 1.47 g CaCl2-2H2O and 10 ml Tween-20 and

mix. 4. Adjust the volume to 1 liter. Store at room

temperature. Storage 20mmol/L Tris-HCl ,200mmol/L NaCL

,2mmol/L CaCl 2 ,50% Glycerol Stored at

-20 ℃ Stability Stable at -20 ℃ , can be used up

to one year

Recombinant Enterokinase

For any questions and needs please contact us below

Company Contact:

  • Phone: 0086057188257817

  • Address: Room3A09, No.452,No.6 street, Hangzhou Economic-Te, Hangzhou, China

  • Email: Email

Published date: September 7, 2012 -

  • Business Description: involved in international trade import export of goods, products, and services

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