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Anti B Raf V600e Mouse Monoclonal Antibody - USA

price: contact company for price

Catalog Number: 26039

Gene Symbol: BRAF, BRAF1, RAFB1

Description: Anti-B-Raf(V600E) Mouse Monoclonal Antibody

Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:2000

IHC: 1:100-1:200

Concentration: 0.5 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2a, IgG2b

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes V600E mutated, but not wild-type B-Raf proteins from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles


Company Contact:


  • Phone: 6109452007

  • Address: 24 Whitewoods Lane, Malvern, PA, USA

  • Email: Email


Published date: June 6, 2013 -

  • Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins). These unique antibodies do not recognize the inactive (GDP bound) G proteins. These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections. Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.

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