Bovine Stem Cell Factor Receptor Scfr Cd117 C Kit Elisa - Chinaprice: contact company for price
Name Stem Cell Factor Receptor, SCFR / CD117/ C- kit
Principles of the Test
This type of assay is called “sandwich” assay because the analyte to be measured is bound between the coating ( capture) antibodies and the detection antibodies. The sandwich format is sensitive and robust. Here, monoclonal BovineCD117 antibodies, showing high specificity toward a single epitope, are used as capture antibody to provide specificity and allow fine detection and quantification of antigens; biotin- conjugated monoclonal Bovine CD117 antibodies, recognizing epitope( s) that are not recognized by capture antibodies, are used as detection antibodies. When the antigen binds to the capture antibody, the epitope recognized by the detection antibody must not be obscured or altered. Capture and detection antibodies that do not interfere with one another and can bind simultaneously are considered a matched pair and are suitable for developing a sandwich ELISA. The biotin- conjugated detection antibodies are further reacted with streptavidin- conjugated HRP- linked enzyme, which can develop a color upon the addition of TMB substrate.
Colorimetric ELISA with TMB substrate for 96- well microplate
Absorbance 450 nm as the primary wave length ( optionally 620 nm as the reference wave length)
No cross- reaction with circulating factors of the immune systeme
Average recovery ranged from80% to 110% with an overall mean recovery of101% .
Yes. Well- to- well. CV < 5%
1) Precoated 96- well Strip Plate
2) 1 bottle Sample Diluent;
3) 1 vial Standard ;
4) 1 vial biotin- conjugated antibody;
5) 1 vial Streptavidin- HRP;
6) 1 bottle Conjugate Diluent;
7) 1 bottle wash buffer
8) 1bottle TMB ( tetramethyl- benzidine) substrate Solution;
9) 1 bottle Stop Solution ;
10) 1 Adhesive Plate Cover.
Total Assay Time
For research use only. Not for diagnostic use
within three days of delivery
- Posted By: Putus Macromolecular Sci. & Tech.ltd
- Phone: 8602787610995
- Address: Room7038, Hi-tech Tower, Huaguang Road 18, Wuhan, China
- Website: http://www.meretciel.com
Published date: June 11, 2014 -
- Business Description: MER & CIELï¼ˆmeretcielï¼‰ is one of the core brands of Putus Macromolecular Sci. & Tech.Ltd (Putus).
Putus is an international
company having three wholly owned subsidiaries Putus, MER & CIELï¼ˆmeretcielï¼‰ and Ingenasa. We
continuously provide products,
consulting service, training and technical support to a wide range of clients in food industries,
stockbreeding and private/governmental
inspection organizations. Our products have been distributed and sold to the Mainland of China, Hong
Kong, Germany, Spain, Finland,
Norway, Sweden, United States and Canada. We have also established long term relationship with our
partners in Asia, Europe and
North America to continuously R&D new product lines.
Putus has developed patented technologies to manufacture high quality carbohydrate and
protein standards of various Mw, while
our subsidiary Ingenasa applies advanced technologies to develop diagnostic kits for animal disease and
food/feed safety. Using these
patented technologies, MER & CIELï¼ˆmeretcielï¼‰ has commercialized over a thousand of products, covering
a wide range of antigens,
monoclonal/polyclonal antibodies and ELISA kits for investigational and clinical research.
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