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  • Diethylstilbestrol Elisa Test Kit - China

    price: contact company for price

    1. Principle

    The BIOKAU® Diethylstilbestrol ELISA Test Kit is based on

    the competitive enzyme immunoassay for the detection of

    Diethylstilbestrol ( DES) in the feed, urine, liver, meat,

    shrimp and fish. The conjugate antigen is pre- coated on the

    micro- well stripes. The Diethylstilbestrol in the sample

    competes with the conjugate antigen pre- coated on the micro-

    well stripes, to interact with the antibodies against

    Diethylstilbestrol. After the addition of the enzyme

    conjugate, the TMB substrate is added for coloration. The

    optical density ( OD) value of the sample has a negative

    correlation with the content of Diethylstilbestrol in the

    sample. This value is compared to the standard curve and the

    content of the corresponding Diethylstilbestrol is

    subsequently obtained.

    2. Technical specifications

    Sensitivity: 0.1 ppb

    Detection limit

    Shrimp,

    fish

    0.2 ppb

    Pork/ liver,

    chicken/ liver.

    . 2 ppb

    Urine.

    0.6 ppb

    Feed

    20 ppb

    Recovery rate

    . 70 ±10%

    90 ±10%

    Tissue

    85 ±10%

    Cross- reactions rate

    DES.

    . 100%

    Dienestrol

    . 38.5%

    Hexestrol.

    . 8.5%

    Ethinylestradiol

    < 0.1%

    Estriol.

    . < 0.1%

    3. Components

    1) Micro- well strips: 12 strips with 8 removable wells

    each

    2) 6 × standard solution ( 1 mL each) : 0 ppb, 0.1 ppb,

    0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

    3) Enzyme conjugate ( 12

    mL) .

    . . . . red cap

    4) Antibody working solution ( 7

    mL) blue

    cap

    5) Substrate A solution ( 7

    . white cap

    6) Substrate B solution ( 7

    mL) .

    black cap

    7) Stop solution ( 7

    . . . . . . . . . yellow cap

    8) 20× concentrated washing buffer ( 40

    mL) . white cap

    9) 5× concentrated redissolving solution ( 50

    mL) . transparent cap

    4. Materials required but not provided

    1) Equipments: microplate reader, printer, homogenizer,

    nitrogen- drying device, votex, centrifuge, measuring pipets,

    balance ( a sensibility reciprocal of 0.01 g)

    2) Micropipettors: single- channel 20- 100 µL , 100- 1000

    µL, and multi- channel 250 µL;

    3) Reagents: NaOH, Acetonitrile ( CH3CN) , Acetone,

    deionized water, H3PO4 ( 85% ) , CHCl3


    Company Contact:


    • Phone: 86013724287324

    • Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen, China

    • Email: Email


    Published date: May 5, 2015 -

    • Business Description: We are a team of experienced scientists with many years of experience on

      antigen, antibody, Gold Colloid and ELISA kit.

      We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.

      BIOKAU provides the most up to date products and technical information available from world class manufacturers, to laboratories, research institutes, life science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.

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