Genomic Shotgun Sequencing - USAprice: contact company for price
plasmids such as BAC, Fosmid and Cosmid, as well as whole genome
shotgun sequencing. In each large-scale sequencing project, a project
manager oversees every step and informs clients about the progress in a
Shotgun library construction
Library quality control by sequencing 96 clones
Clone picking and arraying into 96- or 384-well plate
Plasmid DNA preparation
Large-scale sequencing by ABI 3730xl
Gap closure by primer walking
Genome coverage >99.9%
Template DNA and bioinformatics annotation available
An effective collaboration between bioinformatics and nucleotide departments is essential for successfully reconstructing the original sequence from the reads. In addition to the semi-automated assembly utilizing programs such as Phred, Phrap and Consed, manual curation is applied by senior bioinformaticians to eliminate potential mis-assemblies. Supplementary strategies include primer walking and/or direct PCR sequencing. Paired end DNA sequence information is used to resolve repeat sequences and maximize the level of contig alignment. Furthermore, our in-house annotation pipeline is able to extract and display valuable information from the sequences.
- Posted By: CD Genomics
- Phone: 16316269181
- Address: 45-16 Ramsey Road, Shirley, New York, NY, USA
- Website: http://cd-genomics.com
Published date: May 6, 2013 -
- Business Description: CD Genomics is the biotechnology services company that you can rely on. We specialize in the services of DNA sequencing, genotyping, DNA library construction and aptamer development.
CD Genomics provides the most cost effective high throughput DNA sequencing services with a focus on Shotgun Library Sequencing, cDNA Library Sequencing, SAGE Library Sequencing, Whole Genome Sequencing and Primer Walking Sequencing. Our sequencing facility is equipped with state of the art high throughput sequencers such as ABI 3730xl, 3130xl, Amersham MegaBACE 4000 and 1000. In particular, we have ready access to Roche 454 GS FLX System, ABI SOLiD System and Illumina Solexa 1G Genome Analyzer, the newest technologies in the field. Our sequencing division is well staffed by scientists who have played a key role in a number of large scale genome projects, including Human Genome Project, Rice Genome Project, Silkworm Genome Project, Chicken Genome Project and Porcine Genome Project. In addition, we offer high quality Re sequencing & SNP Discovery Services. Quite a few of our staff scientists once participated in the International HapMap Project too.
In particular, CD Genomics has extensive experience in whole genome shotgun sequencing services. Beginning from bacterial cells or genomic DNA, we provide you with accurate genome consensus sequences. We are also experienced in genome annotation and comparative genomics research. Notably our staff scientists have finished a number of whole microbial genome sequencing projects, such as the genomes of Pseudomonas bathycetes, Bacillus thuringiensis, Thermoanaerobacter tengcongensis, Streptococcus suis, and quite a few virus genomes. In addition, our in house Laboratory Information Management System (LIMS) as well as manual basecalling ensures the highest service quality.
Well equipped with both traditional and mordern genotyping platforms such as Illumina BeadStation 500, Beadxpress and Sequenom, we provide professional services in each and every area of genotyping field with a focus on pharmacogenomics.
CD Genomics has been offering professional DNA library construction services for over 5 years. Hundreds of various cDNA libraries (such as standard cDNA library, large insert cDNA library, normalized cDNA library and subtracted cDNA library) and genomic DNA libraries (including short insert plasmid shotgun library, large insert plasmid shotgun library, whole genome shotgun library, Fosmid library, Cosmid library, MiniBAC and BAC library) have been constructed in our lab and many of them have been sequenced by end sequencing.
CD Genomics has been dedicated to provide a full range of services to develop high quality customized aptamers for research, diagnostic and therapeutic applications. Services offered by CD Genomics include aptamer synthesis, aptamer generation, cell aptamer selection, aptamer design and modification, negative selection and so on.
CD Genomics is headquartered in Shirley, New York with operations in North Carolina as well as internationally in Europe and Asia.
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