Porcine Epidemic Diarrhea Ped Antibody Elisa Kit - Chinaprice: contact company for price
Catalog No. LSY- 30029
This kit is used to detect Porcine Epidemic Diarrhea ( PED) antibody in porcine serum, to assess antibody condition by Porcine Epidemic Diarrhea ( PED) vaccine in porcine farm and assist diagnosis of serological infected porcine.
The Porcine Epidemic Diarrhea ( PED) antibody ELISA kit is based on an indirect enzymatic immunoassay ( Indirect ELISA) . The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer ( 450 nm) .
1) PED antigen coated microtiter strips 1 piece ( 96 wells)
2) Conjugate solution 1 bottle ( 12 mL/ bottle)
3) 10× washing solution1 bottle ( 50 mL/ bottle)
4) Substrate A / B solution 1 tube each ( 6 mL/ tube)
5) Sample dilution 1 bottle ( 50 mL/ bottle)
6) Stopping solution 1 tube ( 6 mL/ tube)
7) Negative control 1 tube ( 800μ L / tube)
8) Positive control 1 tube ( 800μ L / tube)
9) Serum dilution plate 1 piece
10) Adhesive film 2 pieces
11) Instruction 1 piece
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μ L~10μ L、 10μ L~100μ L、 100μ L~1000μ L
2) Disposable pipette tips
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine
5. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, without hemolysis.
- Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
- Phone: 8675528938075
- Address: D building, National Biological Industrial Park of, Shenzhen, China
- Website: http://www.lsybt.com
Published date: July 30, 2014 -
- Business Description: involved in international trade import export of goods, products, and services
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