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Porcine Epidemic Diarrhea Ped Antibody Elisa Kit - China

price: contact company for price

Porcine Epidemic Diarrhea ( PED) Antibody ELISA kit

Catalog No. LSY- 30029

1. Usage

This kit is used to detect Porcine Epidemic Diarrhea ( PED) antibody in porcine serum, to assess antibody condition by Porcine Epidemic Diarrhea ( PED) vaccine in porcine farm and assist diagnosis of serological infected porcine.

2. Principle

The Porcine Epidemic Diarrhea ( PED) antibody ELISA kit is based on an indirect enzymatic immunoassay ( Indirect ELISA) . The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer ( 450 nm) .

3. Reagents

1) PED antigen coated microtiter strips 1 piece ( 96 wells)

2) Conjugate solution 1 bottle ( 12 mL/ bottle)

3) 10× washing solution1 bottle ( 50 mL/ bottle)

4) Substrate A / B solution 1 tube each ( 6 mL/ tube)

5) Sample dilution 1 bottle ( 50 mL/ bottle)

6) Stopping solution 1 tube ( 6 mL/ tube)

7) Negative control 1 tube ( 800μ L / tube)

8) Positive control 1 tube ( 800μ L / tube)

9) Serum dilution plate 1 piece

10) Adhesive film 2 pieces

11) Instruction 1 piece

4. Materials required but not provided

1) Micropipettors and disposable tips: 0.5μ L~10μ L、 10μ L~100μ L、 100μ L~1000μ L

2) Disposable pipette tips

3) Measuring cylinder: 500 ml

4) 96 wells microplate reader

5) Distilled water or deionied water

6) Bottle or microplate washing machine

5. Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, without hemolysis.

Company Contact:

  • Phone: 8675528938075

  • Address: D building, National Biological Industrial Park of, Shenzhen, China

  • Email: Email

Published date: July 30, 2014 -

  • Business Description: involved in international trade import export of goods, products, and services

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