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  • Sell Recombinant Lipase A - China

    price: contact company for price

    Recombinant lipase A

    Biocatalyst with excellent enantioselectivity

    SOURCE:

    E.Coli

    MOL. WEIGHT:

    65 kDa

    FORM:

    Lyophilized powder, Immobilized powder

    DESCRIPTIONS

    Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride.

    APPLICATION

    1)Hydrolyze trans-3-(4-methoxyphynyl) glycidicacid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM].

    2)Hydrolyze (±)-naproxen methyl ester to produce(-)-naproxen.

    3)Hydrolyze Fatty acid esters.

    4)Catalyze turn ester reaction.

    5)Produce biodiesel.

    APPLICATION NOTES

    PH-stability pH5~10

    Thermal-stability Be stable below 40°C, unstable at higher than 50°C.

    Stability against organic solvents Higher activity In 10%-50% DMSO, isopropyl ether, petroleum ether

    In 10%-25% ethanol, acetone and isopropanol,

    In 90% isopropyl ether, petroleum ether

    50% activity In 50% acetone and isopropanol.

    Selectivity to different fatty-acids derives Priory to long-chain (> 10) fatty acids derives.

    APPLICATION EXAMPLES

    Figure 1. HPLC figure of (±)-MPGM using toluence as solvent, lipase A as catalyst. A, (±)-MPGM mixture before reaction; B, adding lipase A for 4h.

    Figure 2. HPLC analysis on lipase A catalize (±)-naproxen methy ester with chiral selectivity

    Figure 3. Selectivity of lipase A on different long-chain fatty acids p-nitrophenyl derives.

    PRODUCTS INFORMATION

    Product Cat.No Activity Packaging Manufacturer

    Recombinant

    Lipase A RLAS063000 ≥3000u/g 10mg 1g bulk YaxinBio

    RLAS062000 ≥2000u/g 10mg 1g bulk YaxinBio

    RLAS06500 ≥500u/g 250mg 10g bulk YaxinBio

    UNIT DEFINITION Lipase was assayed using p-nitrophenyl acetate ( pNPA) as a substrate.

    One unit of lipase activity was defined as the amount of enzyme releasing

    1.0 μmol of p-nitrophenol per minute.

    Sell Recombinant Lipase A

    Feel free to contact us with all your questions


    Company Contact:


    • Phone: 862154336592

    • Address: Room 101, Building 1, No. 466 Yin Du Road, Xu Hui , shanghai, China

    • Email: Email


    Published date: November 16, 2012 -

    • Business Description: involved in international trade import export of goods, products, and services

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