Sell Recombinant Lipase A - Chinaprice: contact company for price
Biocatalyst with excellent enantioselectivity
Lyophilized powder, Immobilized powder
Lipases (EC 184.108.40.206) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride.
1)Hydrolyze trans-3-(4-methoxyphynyl) glycidicacid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM].
2)Hydrolyze (±)-naproxen methyl ester to produce(-)-naproxen.
3)Hydrolyze Fatty acid esters.
4)Catalyze turn ester reaction.
Thermal-stability Be stable below 40°C, unstable at higher than 50°C.
Stability against organic solvents Higher activity In 10%-50% DMSO, isopropyl ether, petroleum ether
In 10%-25% ethanol, acetone and isopropanol,
In 90% isopropyl ether, petroleum ether
50% activity In 50% acetone and isopropanol.
Selectivity to different fatty-acids derives Priory to long-chain (> 10) fatty acids derives.
Figure 1. HPLC figure of (±)-MPGM using toluence as solvent, lipase A as catalyst. A, (±)-MPGM mixture before reaction; B, adding lipase A for 4h.
Figure 2. HPLC analysis on lipase A catalize (±)-naproxen methy ester with chiral selectivity
Figure 3. Selectivity of lipase A on different long-chain fatty acids p-nitrophenyl derives.
Product Cat.No Activity Packaging Manufacturer
Lipase A RLAS063000 ≥3000u/g 10mg 1g bulk YaxinBio
RLAS062000 ≥2000u/g 10mg 1g bulk YaxinBio
RLAS06500 ≥500u/g 250mg 10g bulk YaxinBio
UNIT DEFINITION Lipase was assayed using p-nitrophenyl acetate ( pNPA) as a substrate.
One unit of lipase activity was defined as the amount of enzyme releasing
1.0 μmol of p-nitrophenol per minute.
Sell Recombinant Lipase A
Feel free to contact us with all your questions
- Posted By: China Shanghai Yaxin Biotechnology Co. Ltd
- Phone: 862154336592
- Address: Room 101, Building 1, No. 466 Yin Du Road, Xu Hui , shanghai, China
- Website: http://www.yaxinbio.com
Published date: November 16, 2012 -
- Business Description: involved in international trade import export of goods, products, and services
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