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  • Human Eukaryotic Translation Initiation Factor 3a Eif3a Elisa Kit - China

    price: 180.00 Dollar US$

    PRINCIPLE OF THE ASSAY
    The kit uses a doubleantibody sandwich enzymelinked immunosorbent assay (ELISA) to analyze the level of objective in samples. Add standard and sample to wells precoated with one objective antibody at the same time add second HRPconjugated objective antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The yellow color intensity proportionally correlates to the concentration of the concentration of the objective in samples. SAMPLE COLLECTION AND STORAGES
    Serum - Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 20 minutes at approximately 2000×g. Remove serum and assay immediately or aliquot and store samples at-20℃. Avoid repeated freeze-thaw cycles
    Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 2000×g at 2-8℃within 30 minutes of collection. Store samples at -20℃. Avoid repeated freeze-thaw cycles.
    Cell culture supernates, tissue homogenate and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃. Avoid repeated freeze-thaw cycles.
    Sample preparation - Typically, serum, plasma and other biological fluids samples do not require dilution. If samples generate values higher than the highest standard, please dilute the samples with Sample Diluent and repeat the assay.
    Notes: Serum and plasma to be used within 7 days may be stored at 2-8℃, otherwise samples must be stored at -20 or -80℃ to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles. When performing the assay slowly bring samples to room temperature. The samples shoule be centrifugated dequately and no hemolysis or granule was allowed. REAGENTS PROVIDED
    All reagents provided are stored at 2-8°C. Refer to the expiration date on the label.
    Name 96 determinations 48 determinations
    Microelisa stripplate 12*8strips 12*4strips
    Standard(6 vial) 0.5ml/vial 0.5ml/vial
    Sample diluent 10ml 10ml
    HRP-Conjugate reagent 10ml 5.0ml
    20X Wash solution 25ml 15ml
    Chromogen Solution A 6.0ml 3.0ml
    Chromogen Solution B 6.0ml 3.0ml
    Stop Solution 6.0ml 3.0ml
    Closure plate membrane 2 2
    User manual 1 1
    Sealed bags 1 1


    Company Contact:


    • Phone: +8618252001943

    • Address: kaicheng, nanjing , jiangsu, China

    • Email: Email


    Published date: August 2, 2017 -

    • Business Description: Paris Biochemical Technology Co., Ltd. is a manufacturer and supplier of reagents for research and development. We aims to provide system product resources and supporting technical services for customers in global science ,technology and industrial fields.

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