Anti Ctnnb1 G34v Mouse Monoclonal Antibody - USA

Catalog Number: 26299

Gene Symbol: Beta- catenin, CTNNB, Catenin beta- 1

Description: Anti- CTNNB1 ( G34V) Mouse Monoclonal Antibody

Background: CTNNB1 protein is a dual function protein.

It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells.

CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway.

Mutant CTNNB1 ( ß- catenin) has been implicated in the pathogenesis of several cancers including melanoma, colorectal cancer, hepatocelluar carcinoma, and ovarian cancerMutations of CTNNB1 have been implicated in the pathogenesis of several cancers.

Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of G34V, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:

ELISA: 1: 1000- 1: 2000

WB: 1: 500- 1: 1000

IHC: 1: 50- 1: 100

Concentration: 0.5 mg/ ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G34V mutant, but not wild- type CTNNB1 of vertebrates.

Storage Conditions: Store at - 20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.