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Fumonisin B1 Elisa Kit - China

price: contact company for price

1. Principle

This test kit is based on the competitive enzyme immunoassay

for the detection of Fumonisin B1 in the sample. The

coupling antigen is pre- coated on the micro- well stripes.

The Fumonisin B1 in the sample and the coupling antigens

pre- coated on the micro- well stripes compete for anti-

Fumonisin B1 antibodies. After the addition of the enzyme

conjugate, the TMB substrate is added for coloration. The

optical density ( OD) value of the testing sample has a

negative correlation with the Fumonisin B1 concentration in

the sample. This value is compared to the standard curve and

the Fumonisin B1 concentration is subsequently obtained.

2. Technical specifications

Sensitivity : 10 ppb

Detection limit:

Grain, corn,

feed

0.5 ppm

Linear range:

10ng/ mL~200ng/ mL

Recovery rate:

. 90±15%

Precision:

Intra- assay CV<10% ;

Inter- assay CV<15%

3. Components

1) Micro- well strips: 96wells( 12 strips with 8 removable

wells each)

2) 5× standard solution ( 0.5 mL each) : 0 ppb, 10 ppb, 50

ppb, 100 ppb, 200 ppb

3) Anti- FB1 monoclonal antibody

solution( 6ml) . ready for use directly

4) Enzyme conjugate ( 12

mL) . ready for use

directly

5) Substrate solution ( 12

6) Stop solution ( 6

mL) ready

for use directly

7) 10× concentrated washing buffer ( 30

mL) . dilute for use

4. Materials required but not provided

1) Equipments: microplate reader, oscillator, grinder,

balance( a reciprocal sensibility of 0.01 g) , printer,

centrifuge, measuring pipets, .

2) Material : Graduated cylinder, funnel, flask, rapid

qualitative filter paper

3) Micropipettors: single- channel 20~200 µL, 100~1000

µL; and multi- channel 250 µL;

3) Reagents: 14% Methanol, Sodium chloride ( AR) , deionized

water

5. Sample treatment

Instructions ( The following points must be dealt with before

the pre- treatment )

1) Only the disposable tips can be used for the experiments

and the tips must be changed when used for different

reagents;

2) Before the experiment, each experimental equipment

must be clean and should be re- cleaned if necessary, in

order to avoid the contamination that interferes with the

experimental results.

Make 30ml 70% Methanol solution: such as

21 mL Methanol + 9 mL deionized water ( at volume= 7: 3)

Sample treatment:

1) Take 5g smashed sample into centrifuge, add 1.25g Sodium

chloride ( AR) and 25 mL 70% Methanol solution, shake

severely for 3 min.

2) Filter at rapid qualitative filter paper.

3) Take 1mL filtrate, add 9 mL1% NaCl solution for diluting.

4) Take 50 µL for analysis.

Fold of dilution of sample : 50


Company Contact:


  • Phone: 86013724287324

  • Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen, China

  • Email: Email


Published date: May 5, 2015 -

  • Business Description: We are a team of experienced scientists with many years of experience on

    antigen, antibody, Gold Colloid and ELISA kit.

    We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.

    BIOKAU provides the most up to date products and technical information available from world class manufacturers, to laboratories, research institutes, life science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.

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