Fumonisin B1 Elisa Kit - Chinaprice: contact company for price
This test kit is based on the competitive enzyme immunoassay
for the detection of Fumonisin B1 in the sample. The
coupling antigen is pre- coated on the micro- well stripes.
The Fumonisin B1 in the sample and the coupling antigens
pre- coated on the micro- well stripes compete for anti-
Fumonisin B1 antibodies. After the addition of the enzyme
conjugate, the TMB substrate is added for coloration. The
optical density ( OD) value of the testing sample has a
negative correlation with the Fumonisin B1 concentration in
the sample. This value is compared to the standard curve and
the Fumonisin B1 concentration is subsequently obtained.
2. Technical specifications
Sensitivity : 10 ppb
10ng/ mL~200ng/ mL
Intra- assay CV<10% ;
Inter- assay CV<15%
1） Micro- well strips: 96wells( 12 strips with 8 removable
2） 5× standard solution ( 0.5 mL each) : 0 ppb, 10 ppb, 50
ppb, 100 ppb, 200 ppb
3） Anti- FB1 monoclonal antibody
solution( 6ml) . ready for use directly
4） Enzyme conjugate ( 12
mL) . ready for use
5） Substrate solution ( 12
6） Stop solution ( 6
for use directly
7） 10× concentrated washing buffer ( 30
mL) . dilute for use
4. Materials required but not provided
1) Equipments: microplate reader, oscillator, grinder,
balance( a reciprocal sensibility of 0.01 g) , printer,
centrifuge, measuring pipets, .
2) Material : Graduated cylinder, funnel, flask, rapid
qualitative filter paper
3) Micropipettors: single- channel 20~200 µL, 100~1000
µL; and multi- channel 250 µL;
3) Reagents: 14% Methanol, Sodium chloride ( AR) , deionized
5. Sample treatment
Instructions ( The following points must be dealt with before
the pre- treatment )
1) Only the disposable tips can be used for the experiments
and the tips must be changed when used for different
2) Before the experiment, each experimental equipment
must be clean and should be re- cleaned if necessary, in
order to avoid the contamination that interferes with the
Make 30ml 70% Methanol solution: such as
21 mL Methanol + 9 mL deionized water ( at volume= 7: 3)
1) Take 5g smashed sample into centrifuge, add 1.25g Sodium
chloride ( AR) and 25 mL 70% Methanol solution, shake
severely for 3 min.
2) Filter at rapid qualitative filter paper.
3) Take 1mL filtrate, add 9 mL1% NaCl solution for diluting.
4) Take 50 µL for analysis.
Fold of dilution of sample : 50
- Posted By: Shenzhen Biokau Sci-tech Co.,ltd
- Phone: 86013724287324
- Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen, China
- Website: http://www.biokau.com
Published date: May 5, 2015 -
- Business Description: We are a team of experienced scientists with many years of experience on
antigen, antibody, Gold Colloid and ELISA kit.
We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.
BIOKAU provides the most up to date products and technical information available from world class manufacturers, to laboratories, research institutes, life science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.
High Range Additive Concrete Pce Superplasticizer AdmixtureChemicals - Reagents - Henan Thinkhigh Material Technologies Co., Ltd. - China - May 20, 2016 - contact company for price
High range additive concrete PCE superplasticizer Admixture contains polycarboxylate ether polymers as well as performs outstanding high water reduction and significantly reduces slump loss. With the design for the need to improve the initial compres...
Human Interleukin 1 Il Elisa KitChemicals - Reagents - Shanghai YL Biotech Co. Ltd - China - May 6, 2016 - contact company for price
Method: sandwich Detection range: 1ng/ Lâ†’300ng/ L Sensitivity: 0.52ng/ L Assay length: 1- 5Hours Detection Wavelengt: 450nm Research Area : Immunology Price: inquiry Quality guarantee period: for 12 months We provide a wide range of ELISA KITS, ANT...
1ml Auto Sample VialChemicals - Reagents - Ningbo Excellent New Materials Co Ltd - China - May 4, 2016 - contact company for price
Product Features: Suitable for Agilent, Waters, varian, Shimadzu and other autosamplers . assembled with cap and septa , use them instantly to save your time. With Writing pad , convenient for pencil marks. optional. Precise bottle neck size, ensure ...
Safe Trade Tips
- Ask for business references and check them
- Use a payment method that offers better protection for all such as Letters of credit and verified professional escrow services. Avoid payments in advance such as money transfers
- Verify their business via their local Chamber of Commerce
- Search the internet using their website address, their business name, their phone and fax numbers, and their email addresses to see if you can find any feedback about them.
- Get to know more about scams to avoid: Internet Crime Complaint Center - International Financial Scams
- This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"