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  • Human Interleukin 1beta Elisa Kit - China

    price: check with company for price

    Method:sandwich
    Detection range:20pg/L→6000pg/L
    Sensitivity:10.07pg/L
    Assay length:1-5Hours
    Detection Wavelengt:450nm
    Research Area:Immunology
    Price:inquiry
    Quality guarantee period:for 12 months
    Introduction

    Reagents
    Quantity
    Reagents
    Quantity
    Coated ELISA plate
    12-Well * 8 Tubes
    Washing concentrate (30X)
    20ml
    Standard dilution
    3ml
    Instruction
    1
    Chromogen solution A
    6ml
    Seal plate membrane
    2
    Chromogen solution B
    6ml
    Hermetic bag
    1
    Streptavidin-HRP
    6ml
    Stop solution
    6ml
    Standard solution
    0.5ml
    Antibodies labeled with biotin
    1ml

    INTENDED USE
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

    STORAGE
    For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃

    PRECISION
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<8%
    Inter-Assay: CV<10%

    ASSAY PROCEDURE SUMMARY
    1. Prepare all reagents, samples and standards;
    2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
    3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.
    4. Add stop solution
    5. Read the OD value within 10minutes.
    6. Calculate


    Company Contact:


    • Phone: 8615057279866

    • Address: No.5588,Caoan Road,Jiading District,Shanghai,China, Shanghai, China

    • Email: Email


    Published date: November 7, 2016 -

    • Business Description: YL Biont is a collection of research, production, sales as one of the

      leading international biological high-tech enterprise.

      the company focused on the immune detection reagents of independent research and development, the main products include ELISA kit, CLIA kit, protein, antibody, mark kit and other related reagents, which a total of more than 20000 products! Through the national support and new high-tech industries promoted, we has been the advanced production technology in the field of biological kits. YL company will continue to research and develop, improve competition of products quality. At the same time, the products of the research and development face the market at home and abroad.

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