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Human Interleukin 1beta Elisa Kit - China

price: check with company for price

Method:sandwich
Detection range:20pg/L→6000pg/L
Sensitivity:10.07pg/L
Assay length:1-5Hours
Detection Wavelengt:450nm
Research Area:Immunology
Price:inquiry
Quality guarantee period:for 12 months
Introduction

Reagents
Quantity
Reagents
Quantity
Coated ELISA plate
12-Well * 8 Tubes
Washing concentrate (30X)
20ml
Standard dilution
3ml
Instruction
1
Chromogen solution A
6ml
Seal plate membrane
2
Chromogen solution B
6ml
Hermetic bag
1
Streptavidin-HRP
6ml
Stop solution
6ml
Standard solution
0.5ml
Antibodies labeled with biotin
1ml

INTENDED USE
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

STORAGE
For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃

PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<10%

ASSAY PROCEDURE SUMMARY
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.
6. Calculate


Company Contact:


  • Phone: 8615057279866

  • Address: No.5588,Caoan Road,Jiading District,Shanghai,China, Shanghai, China

  • Email: Email


Published date: November 7, 2016 -

  • Business Description: YL Biont is a collection of research, production, sales as one of the

    leading international biological high-tech enterprise.

    the company focused on the immune detection reagents of independent research and development, the main products include ELISA kit, CLIA kit, protein, antibody, mark kit and other related reagents, which a total of more than 20000 products! Through the national support and new high-tech industries promoted, we has been the advanced production technology in the field of biological kits. YL company will continue to research and develop, improve competition of products quality. At the same time, the products of the research and development face the market at home and abroad.

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