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  • Human Pcna Proliferating Cell Nuclear Antigen Elisa Kit - China

    price: contact company for price

    Product name

    Human PCNA ( Proliferating Cell Nuclear Antigen) ELISA Kit

    Catalog No.

    E- EL- H2399

    Package

    96T/ kit

    Detection Range

    0.313- 20ng/ mL

    Sensitivity

    0.188 ng/ mL

    Specificity

    This kit recognizes natural and recombinant Human PCNA. No

    significant cross- reactivity or interference between Human

    PCNA and analogues was observed. Note: Limited by existing

    techniques, cross- reaction may still exist, as it is

    impossible for us to complete the cross- reactivity detection

    between Human PCNA and all the analogues.

    Repeatability

    Coefficient of variation were<10% .

    Test principle

    This ELISA kit uses Sandwich- ELISA as the method. The micro

    ELISA plate provided in this kit has been pre- coated with an

    antibody specific to Human PCNA. Standards or samples are

    added to the appropriate micro ELISA plate wells and bound

    by the specific antibody. Then a biotinylated detection

    antibody specific for Human PCNA and Avidin- Horseradish

    Peroxidase ( HRP) conjugate is added to each micro plate well

    successively and incubated. Free components are washed away.

    The substrate solution is added to each well. Only those

    wells that contain Human PCNA, biotinylated detection

    antibody and Avidin- HRP conjugate will appear blue in color.

    The enzyme- substrate reaction is terminated by the addition

    of a sulphuric acid solution and the color turns yellow. The

    optical density ( OD) is measured spectrophotometrically at a

    wavelength of 450 nm ± 2 nm. The OD value is proportional

    to the concentration of Human PCNA. You can calculate the

    concentration of Human PCNA in the samples by comparing the

    OD of the samples to the standard curve.


    Company Contact:


    • Phone: 862787385095

    • Address: No 1, Shizishan Street, Hongshan District , Wuhan, China

    • Email: Email


    Published date: April 18, 2014 -

    • Business Description: Elabscience ELISA Kits supplier offering 20,000 kind of

      products covering the following series:Cytokine, Signal

      transduction, CD & Adhesion molecules, Metabolism, Endocrinium, Innate

      immune, Infection immunity ,Tumor immune, Nervous system, Hematological

      system, Urinary system.

      We are committed to offering the best products and services for our customers. For more information,please visit our website.

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