59 visiting now: 29 China China flag 15 France France flag 8 USA USA flag 2 Germany Germany flag 2 Netherlands Netherlands flag 2 Sweden Sweden flag 1 UK UK flag

Recombinant Chymotrypsin - China

price: contact company for price

Recombinant Chymotrypsin

Animal Protein Free, No Virus Contaminant


Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.


● Animal origin free

The use of recombinant chymotrypsin eliminates the content of other proteins, the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

● Stable

A sterile recombinant chymotrypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

● High purity

1)Recombinant Chymotrypsin provides increased specificity and eliminates contaminating activities found in enzymes purified from pancreas.

2) No anion trypsin or cation trypsin.

3) One single main band at 25kD by SDS-PAGE.


Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-termini of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids.


Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.

For peptide digestion, use a ratio (w/w) of approximately 1:60 for chymotrypsin:peptide. Perform peptide digests in 100 mM Tris HCl containing 10 mM CaCl2, pH 7.8, at 30 °C. Self digestion may occur if temperatures above 37 °C are used. A known peptide such as melittin should be used as a control for all experiments.


Source E. Coli

Purified by HPLC

Physical form White lyophilized powder

Specific activity ≥1000 unit/mg pro.

Purity > 95%


Product Cat. No. Specific activity Packaging Manufacturer

Recombinant Chymotrypsin RCT10 ≥1000 unit/mg pro. 1mg, 100mg, 1g,

bulk YaxinBio

UNIT DEFINITION: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

RELATED PRODUCT: Recombinant Trypsin, V8

Company Contact:

  • Phone: 862154336592

  • Address: Room 101, Building 1, No. 466 Yin Du Road, Xu Hui , shanghai, China

  • Email: Email

Published date: January 25, 2014 -

  • Business Description: involved in international trade import export of goods, products, and services

Related listings

  • Sodium Lignosulphonate Mn 2
    Sodium Lignosulphonate Mn 2
    Chemicals - Reagents - Hubei Aging Chemical Co.,Ltd - China - December 2, 2013 - contact company for price

    Sodium Lignosulphonate ( lignosulfonate ) water reducer are mainly for concrete mixture as water-reducing additive. Low dosage , low air content , water reducing rate is high , adapt to most kind of cement. Can confected as concrete early-age strengt...

  • Rosarin Cas No 84954 93 8
    Rosarin Cas No 84954 93 8
    Chemicals - Reagents - Chengdu Must Bio-Technology Co.,Ltd - China - November 20, 2013 - contact company for price

    Product No.:A0077 Name:Rosarin Alias:(2E)-3-phenyl-2-propenyl6-O-.alpha.-L-arabinofuranosyl-(9CI) CAS No.:84954-93-8 Formula:C20H28O10 Formula Weight:428.43 Exterior:Supplying Package:20mg/pc Purity:≥98% by HPLC Source extraction:Rhodiola rosea...

  • Rosavin Cas No 84954 92 7
    Rosavin Cas No 84954 92 7
    Chemicals - Reagents - Chengdu Must Bio-Technology Co.,Ltd - China - November 20, 2013 - contact company for price

    Product No.:A0078 Name:Rosavin Alias:Supplying…. CAS No.:84954-92-7 Formula:C20H28O10 Formula Weight:428.43 Exterior:Supplying Package:20mg/pc Purity:≥98% by HPLC Source extraction:Rhodiola Valid:2 years Capacity:In stock, customized for large ...

Safe Trade Tips

  • Ask for business references and check them
  • Use a payment method that offers better protection for all such as Letters of credit and verified professional escrow services. Avoid payments in advance such as money transfers
  • Verify their business via their local Chamber of Commerce
  • Search the internet using their website address, their business name, their phone and fax numbers, and their email addresses to see if you can find any feedback about them.
  • Get to know more about scams to avoid: Internet Crime Complaint Center - International Financial Scams
  • This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"