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  • Anti Braf D594g Mouse Monoclonal Antibody - USA

    price: contact company for price

    Catalog Number: 26200

    Gene Symbol: BRAF, BRAF1, RAFB1

    Description: Anti- BRAF ( D594G) Mouse Monoclonal Antibody

    Background: BRAF belongs to a family of serine- threonine protein kinases. As a part of a signaling pathway known as the the RAS/ MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

    Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.

    Applications: ELISA, WB

    Recommended Dilutions:

    ELISA: 1: 1000- 1: 5000

    WB: 1: 500- 1: 1000

    Concentration: 1 mg/ ml

    Host: Mouse

    Format: Liquid

    Clonality: Monoclonal

    Isotype:

    Purity: Purified from ascites

    Storage buffer:

    Preservative: no

    Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

    Species Reactivity: Recognizes D594G mutant, but not wild- type BRAF of vertebrates.

    Storage Conditions: Store at - 20°C. Avoid freeze / thaw cycles


    Company Contact:


    • Phone: 6109452007

    • Address: 24 Whitewoods Lane, Malvern, PA, USA

    • Email: Email


    Published date: May 4, 2016 -

    • Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins). These unique antibodies do not recognize the inactive (GDP bound) G proteins. These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections. Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.

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