Anti Rheb Y35n Mouse Monoclonal Antibody - USA

Catalog Number: 26338

Gene Symbol: RHEB

Description: Anti- RHEB ( Y35N) Mouse Monoclonal Antibody

Background: RheB ( Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras- family of small GTP- binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure.

While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation.

RheB has an important role in regulating the insulin/ TOR signaling pathway.

The mammalian target of rapamycin, mTOR, is a serine/ threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth.

The tuberin/ hamartin ( TSC2/ TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin' s GAP activity.

Immunogen: A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:

ELISA: 1: 1000- 1: 2000

WB: 1: 500- 1: 1000

IHC: 1: 50- 1: 100

Concentration: 1 mg/ ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Y35N mutant, but not wild- type RHEB of vertebrates.

Storage Conditions: Store at - 20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.