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946 Agonists Elisa Test Kit - China

price: contact company for price

1. Principle

The BIOKAU®β - agonists test kit is based on the competitive

enzyme immunoassay for the detection of β - agonists in the

urine, pork and other samples. The coupling antigen is pre-

coated on the micro- well stripes. The β - agonists in the

sample and the coupling antigens pre- coated on the micro-

well stripes compete for the anti- β - agonists antibodies.

After the addition of the enzyme conjugate, the TMB

substrate is added for coloration. The optical density ( OD)

value of the sample has a negative correlation with the β -

agonists in the sample. This value is compared to the

standard curve and the β - agonists residues is subsequently

obtained.

2. Technical specifications

Sensitivity: 0.3 ppb

Detection limit:

Tissue

0.3 ppb

Urine,

serum.

Feed

. 30 ppb

Recovery rate:

90±10%

80±10%

80±15%

Cross- reaction rate:

Clenbuterol.

. 100%

Salbutamol

120%

Bromchlorbuterol

. 130%

Brombuterol.

. 90%

Mabuterol.

. 70%

Clenpenterol

65%

Mapenterol

55%

Tebutalin.

42%

Carbuterol

. 35%

Clenproperol

. 15%

Cimaterol.

12%

Dobutamine

<3%

Ritodrine.

<1%

Ractopamine.

. <0.5%

Precision:

For the intra- assay precision, CV<8%

For the inter- assay precision, CV<10%

3. Components

1) Micro- well strips: 12 strips with 8 removable wells

each

2) 6× standard solution ( 1 mL each) : 0 ppb, 0.3ppb, 0.9

ppb, 2.7 ppb, 8.1 ppb, 25 ppb

3) Enzyme conjugate ( 7

mL) .

. . . . . . red cap

4) Antibody working solution ( 10

mL) blue cap

5) Substrate A solution ( 7

mL) .

white cap

6) Substrate B solution ( 7

black cap

7) Stop solution ( 7

. . . . . . . . . yellow cap

8) 20× concentrated washing buffer ( 40

mL) . white cap

9) 2× concentrated redissolving solution ( 50

mL) . transparent cap

4. Materials required but not provided

1) Equipments: microplate reader, printer, homogenizer,

nitrogen- drying device, vortex, oscillator, centrifuge,

measuring pipets, balance ( a sensibility reciprocal of 0.01

g)

2) Micropipettors: single- channel 20- 200 µL, 100- 1000

µL, and multi- channel 250 µL;

3) Reagents: Acetonitrile ( CH3CN) , NaOH, ethyl acetate,

HCI ( approx 36.5% ) , N- hexane, isopropanol( C3H8O)

5. Sample pre- treatment

Solution preparation before sample pre- treatment:

1) 1 M HCI: dissolve 8.6 mL HCI ( approx 36.5% ) in

deionized water to 100 mL.

2) 1 M NaOH: dissolve 4 g NaOH in deionized water to

100 mL.

3) Dillute 2× concentrated redissolving solution with

deionized water at 1: 1( 1× concentrated redissolving

solution, used for sample redissolving) and 1: 3( 0.5×

concentrated redissolving solution, used for extraction of

tissue sample)


Company Contact:


  • Phone: 86013724287324

  • Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen, China

  • Email: Email


Published date: May 5, 2015 -

  • Business Description: We are a team of experienced scientists with many years of experience on

    antigen, antibody, Gold Colloid and ELISA kit.

    We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.

    BIOKAU provides the most up to date products and technical information available from world class manufacturers, to laboratories, research institutes, life science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.

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