Total Aflatoxins Elisa Test Kit - Chinaprice: contact company for price
Aflatoxins are secondary metabolites of the fungi species
Aspergillus flavus, parasiticus and nomius. Aflatoxins
belong to the strongest natural occurring carcinogenic
substances, commonly found in corn, peanuts, cotton seed,
human blood and animal feed.
2. Test principle
This test kit is based on the competitive enzyme immunoassay
for the detection of AFLATOXINS in the sample. The coupling
antigens are pre- coated on the micro- well stripes. The
AFLATOXINS in the sample and the coupling antigens pre-
coated on the micro- well stripes compete for the anti-
AFLATOXINS antibodies. After the addition of the enzyme
conjugate, the TMB substrate is added for coloration. The
optical density ( OD) value of the sample has a negative
correlation with the AFLATOXINS in it. This value is
compared to the standard curve and the AFLATOXINS
concentration is subsequently obtained.
This test kit can be used to detect total aflatoxins in
maize, corn, feed, edible oil and other samples
qualitatively and quantitatively .
4. Reagents provided
Each kit contains sufficient materials for 96 measurements
( including standard analyses) . Each test kit contains:
1 x Microtiter plate ( 12 strips with 8 removable wells each)
coated with capture antigen
6 x standards, 1 ml each: 0 ppb ( zero standard) , 0.1 ppb,
0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
1 x High concentration standard, 1ml 100ppb
1 x Enzyme
. . . . . . 10 ml
1 x Antibody working
solution. 6 ml
1 x Substrate
. . . . . . . 10ml
1 x Stop
1 x Concentrated washing
5. Reagents required but not provided
− microtiter plate spectrophotometer ( 450 nm/ 630nm)
− vortex Shaker
− water bath
− blance: 0.01g quantity sensitive
− gaduated pipettes: 10ml
− rubber suction bulb
− polystyrene centrifuge tube: 10ml, 50ml
− filter funnel
− Rotary evaporator/ nitrogen evaporator
− Glass Centrifuge Tube: 10ml
− Variable 20 μ l - 200 μ l und 200 - 1000 μ l
− distilled or deionized water
− petroleum ether / n- hexane
a. washing buffer
Use 1 part of concentrated washing buffer and dissolve with
9 parts of distilled water to obtain the ready to use
washing buffer. Solution is stable for 1 months when stored
at 4 °C.
b. 60/ 40 ( v/ v) MeOH/ Water
Add 600 mL MeOH to 400 mL distilled water.
c. 70/ 30 ( v/ v) MeOH/ Water
Add 700 mL MeOH to 300 mL distilled water.
7. Samples Preparation
7.1 Sample Preparation of peanut
− weigh 10 g of ground sample into a suitable container and
add 30 ml of 60/ 40 ( v/ v) MeOH/ Water
− shake vigorously for five minutes ( manually or with
− filter the extract through filter or centrifuge at
3500r/ min for 5 minutes.
− dilute 1 ml of the obtained filtrate with 5 ml of
− use 50 μ l of the diluted filtrate for test
Dilution factor: 18
7.2 Preparation of animal feed and corn sample
− weigh 3 g of ground sample into a suitable container and
add 15 ml of 70/ 30 ( v/ v) MeOH/ Water.
− shake vigorously for 10 minutes ( manually or with shaker)
− dilute 1 ml of the obtained filtrate or suppernant with 6
ml of distilled water.
Dilution factor: 35
7.3 Preparation of edible oil
− weigh 5 g of edible oil sample into a beaker
− Transfer the sample into a 125ml separating funnel with
10ml petroleum ether / n- hexane
− Add 15ml 60/ 40 ( v/ v) MeOH/ Water, cover tightly and shake
for 5min. Put it aside for layering and then release the
lower methanol extract
− dilute 1 ml of the obtained extract with 5 ml of
distilled water/ deionized water.
− use 50 μ l of the diluted extract for test
8. Warnings and precautions for the users
a. The standard solutions contain aflatoxin B1, particular
care should be taken. Avoid contact of the reagent with the
skin ( use gloves) .
b. Decontamination of glassware and aflatoxin B1 solutions
is best carried out using a sodium hypochlorite ( bleach)
solution ( 10 % ( v/ v) ) , adjust solution with HCl to pH 7)
e. The stop solution contains 2 M sulfuric acid.
- Posted By: Shenzhen Biokau Sci-tech Co.,ltd
- Phone: 86013724287324
- Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen, China
- Website: http://www.biokau.com
Published date: May 5, 2015 -
- Business Description: We are a team of experienced scientists with many years of experience on
antigen, antibody, Gold Colloid and ELISA kit.
We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.
BIOKAU provides the most up to date products and technical information available from world class manufacturers, to laboratories, research institutes, life science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.
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