946 Agonist Elisa Kit - China

β-agonists ELISA Test Kit

Catalog No.

LSY-10036

1 - Principle

The GreenSpring®β-agoni sts test kit is based on the competitive enzyme immunoassay for the detection of β-agonists in the sample.

The coupling antigen is pre-coated on the micro-well stripes.

The β-agonists in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-agonists antibodies.

After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the β-agonists in the sample.

This value is compared to the standard curve and the β-agonists residues is subsequently obtained.

2 - Technical specifications

Sensitivity: 0.2 ppb

Incubation Temperature: 25℃

Incubation Time: 15min

Detection limit:

Urine 0.2 ppb

Tissue (Method one) 0.8 ppb

Tissue (Method two) 0.2 ppb

Feed 2 ppb

Cross-reaction rate:

Clenbuterol 100%

Salbutamol 120%

Bromchlorbuterol 130%

Brombuterol 90%

Mabuterol 70%

Clenpenterol 65%

Mapenterol 55%

Tebutalin 42%

Carbuterol 35%

Clenproperol 15%

Cimaterol 12%

Dobutamine <3%

Ritodrine <1%

Ractopamine <0.5%

Recovery rate:

Urine, serum 95%±20%

Tissue 85%±25%

Feed 85%±23%

3 - Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.2ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb

3） Enzyme conjugate (10 mL) red cap

4） Antibody working solution (12 mL) blue cap

5） Substrate A solution (7 mL) white cap

6） Substrate B solution (7 mL) black cap

7） Stop solution (7 mL) yellow cap

8） 20X sample extract solution (50 ml)…………………⠀¦â€¦â€¦â€¦.

…….

blue cap

9） 20× concentrated washing buffer (40 mL) white cap

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services