Sulfonamides Residues Sas Elisa Test Kit - China

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Sulfonamides (SAs) ELISA Kit

Catalog No.

LSY-10009

1 - Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides in the chicken, pork, milk, honey, egg, etc.

The coupling antigens are pre-coated on the micro-well stripes.

The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies.

After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample.

This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.

2 - Technical specifications

Sensitivity: 0.4 ppb

Detection limit

Tissue (high-detection-limit method) 0.4 ppb

Tissue (lower-detection-limit method)……………… …………………….

.

2 ppb

Honey, egg …0.4 ppb

Serum, urine 1.6 ppb

Milk 8 ppb

Recovery rate

Tissue, honey 90 ±10%

Egg 65 ±10%

Urine, milk, serum 70 ±10%

Cross-reaction rate

Sulfadiazine (SD or SDZ) 100%

Sulfamonomethoxine (SMM) 97.6%

Sulfamethoxazole (SMZ) 100%

Sulfathiazole (ST) 195.0%

Sulfamerazine (SM1) 92%

Phthalylsulfathiazole (PST) 73%

Sulfamethyltiadiazolum 110%

Sulfapyridine 48%

Sulfamethoxydiazine(SM D) 26.4%

Sulfafurazole(SIZ) 95.0%

Sulfanethazine(SM2)… .

140%

Sulfadimethoxine(SDM) 110%

Sulfadoxine (SDM2).

80%

Sulfaquinoxaline(SQX)� ��160%

Sulfamethoxypyridazine (SMP)………………� �…………………… ………80%

Sulfachlorinepyridazin e (SPDZ)………………� ��…………………� �…….

75%

Sulfabenzoyl (SML).

104%

Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides.

3 - Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.4 ppb, 1.2 ppb, 3.6 ppb, 10.8 ppb and 32.4 ppb

3） Enzyme conjugate (7 mL) red cap

4） Antibody working solution (10 mL) blue cap

5） Substrate A solution (7 mL) white cap

6） Substrate B solution (7 mL) black cap

7） Stop solution (7 mL) yellow cap

8） 20× concentrated washing buffer (40 mL) white cap

9） 2× concentrated redissolving solution (50 mL) transparent cap

Sulfonamides Residues Sas Elisa Test Kit

Please inquiry for your need now

Company Contact:

Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

Phone: 8675528938075

Address: D building, National Biological Industrial Park of, Shenzhen , China

Email:

Website: https://www.lsybt.com

Published date: December 5, 2012

Business Description: involved in international trade import export of goods, products, and services

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