Anti Idh2 R172k Mouse Monoclonal Antibody - USA

Catalog Number: 26163

Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description: Anti-IDH2 (R172K) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.

The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3 - IDH2 plays a role in intermediary metabolism and energy production.

Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2).

Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.

Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:

ELISA: 1: 1000-1: 5000

WB: 1: 100-1: 1000

IHC: 1: 50-1: 100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: recognizes R172K mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.