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Monoclonal Anti Cgmp Antibody Based Direct Eia Kit Without Acetylation - USA
price: contact company for price
Competitive immunoassay to measure the cGMP levels, either from cell
extracts or from in vitro guanylyl cyclase assays.
Briefly, multi-well
plates are coated with goat-anti-mouse serum.
CGMP in cell extracts or
in in vitro guanylyl cyclase assays will competitively bind to the
monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-
conjugated horse-radish peroxidase.
Known amounts of cGMP are used as
standards to generate the calculation curve.
After a short incubation,
the excess reagents are washed away and substrate is added.
The
multiwell plates are then read on a microplate reader at 450 nm.
The
intensity of the yellow color is inversely proportional to the
concentration of cGMP in samples.
The measured optical density is used
to calculate the concentration of cGMP in samples based on the curve
from the cGMP standards.
Company Contact:
- Posted By: NewEast Biosciences
- Phone: 6109452007
- Address: 24 Whitewoods Lane, Malvern , PA , USA
- Email:
- Website: http://www.neweastbio.com
Published date: June 6, 2013
- Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
These unique antibodies do not recognize the inactive (GDP bound) G proteins.
These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.
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