Porcine Epidemic Diarrhea Ped Antibody Elisa Kit - China

Porcine Epidemic Diarrhea ( PED) Antibody ELISA kit

Catalog No.

LSY- 30029

1 - Usage

This kit is used to detect Porcine Epidemic Diarrhea ( PED) antibody in porcine serum, to assess antibody condition by Porcine Epidemic Diarrhea ( PED) vaccine in porcine farm and assist diagnosis of serological infected porcine.

2 - Principle

The Porcine Epidemic Diarrhea ( PED) antibody ELISA kit is based on an indirect enzymatic immunoassay ( Indirect ELISA) .

The antigen is coated on plates.

When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates.

Wash the unbound antibodies and other components.

Then add a specific enzyme conjugate.

After incubation and washing, add the TMB substrate.

A colorimetric reaction will appear, measured by a spectrophotometer ( 450 nm) .

3 - Reagents

1) PED antigen coated microtiter strips 1 piece ( 96 wells)

2) Conjugate solution 1 bottle ( 12 mL/ bottle)

3) 10× washing solution1 bottle ( 50 mL/ bottle)

4) Substrate A / B solution 1 tube each ( 6 mL/ tube)

5) Sample dilution 1 bottle ( 50 mL/ bottle)

6) Stopping solution 1 tube ( 6 mL/ tube)

7) Negative control 1 tube ( 800μ L / tube)

8) Positive control 1 tube ( 800μ L / tube)

9) Serum dilution plate 1 piece

10) Adhesive film 2 pieces

11) Instruction 1 piece

4 - Materials required but not provided

1) Micropipettors and disposable tips: 0.5μ L~10μ L、 10μ L~100μ L、 100μ L~1000μ L

2) Disposable pipette tips

3) Measuring cylinder: 500 ml

4) 96 wells microplate reader

5) Distilled water or deionied water

6) Bottle or microplate washing machine

5 - Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, without hemolysis.

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services