Fumonisin B1 Elisa Kit - China

1 - Principle

This test kit is based on the competitive enzyme immunoassay

for the detection of Fumonisin B1 in the sample.

The

coupling antigen is pre- coated on the micro- well stripes.

The Fumonisin B1 in the sample and the coupling antigens

pre- coated on the micro- well stripes compete for anti-

Fumonisin B1 antibodies.

After the addition of the enzyme

conjugate, the TMB substrate is added for coloration.

The

optical density ( OD) value of the testing sample has a

negative correlation with the Fumonisin B1 concentration in

the sample.

This value is compared to the standard curve and

the Fumonisin B1 concentration is subsequently obtained.

2 - Technical specifications

Sensitivity : 10 ppb

Detection limit:

Grain, corn,

feed

0.5 ppm

Linear range:

10ng/ mL~200ng/ mL

Recovery rate:

.

90±15%

Precision:

Intra- assay CV<10% ;

Inter- assay CV<15%

3 - Components

1） Micro- well strips: 96wells( 12 strips with 8 removable

wells each)

2） 5× standard solution ( 0.5 mL each) : 0 ppb, 10 ppb, 50

ppb, 100 ppb, 200 ppb

3） Anti- FB1 monoclonal antibody

solution( 6ml) .

ready for use directly

4） Enzyme conjugate ( 12

mL) .

ready for use

directly

5） Substrate solution ( 12

6） Stop solution ( 6

mL) ready

for use directly

7） 10× concentrated washing buffer ( 30

mL) .

dilute for use

4 - Materials required but not provided

1) Equipments: microplate reader, oscillator, grinder,

balance( a reciprocal sensibility of 0.01 g) , printer,

centrifuge, measuring pipets, .

2) Material : Graduated cylinder, funnel, flask, rapid

qualitative filter paper

3) Micropipettors: single- channel 20~200 µL, 100~1000

µL; and multi- channel 250 µL;

3) Reagents: 14% Methanol, Sodium chloride ( AR) , deionized

water

5 - Sample treatment

Instructions ( The following points must be dealt with before

the pre- treatment )

1) Only the disposable tips can be used for the experiments

and the tips must be changed when used for different

reagents;

2) Before the experiment, each experimental equipment

must be clean and should be re- cleaned if necessary, in

order to avoid the contamination that interferes with the

experimental results.

Make 30ml 70% Methanol solution: such as

21 mL Methanol + 9 mL deionized water ( at volume= 7: 3)

Sample treatment:

1) Take 5g smashed sample into centrifuge, add 1.25g Sodium

chloride ( AR) and 25 mL 70% Methanol solution, shake

severely for 3 min.

2) Filter at rapid qualitative filter paper.

3) Take 1mL filtrate, add 9 mL1% NaCl solution for diluting.

4) Take 50 µL for analysis.

Fold of dilution of sample : 50

Company Contact:

• Posted By: Shenzhen Biokau Sci-tech Co.,ltd

• Phone: 86013724287324

• Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen , China

• Email:

• Website: http://www.biokau.com

• Business Description: We are a team of experienced scientists with many years of experience on
  
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