Anti Mek1 E203k Mouse Monoclonal Antibody - USA

Catalog Number: 26268

Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

Description: Anti- MEK1 ( E203K) Mouse Monoclonal Antibody

Background: MEK1 ( also known as extracellular signal- regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation.

Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ ERK1 and MAPK1/ ERK2, thus activates the MAP pathway and regulates transcription.

Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome ( CFC) and melanoma.

Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of E203K, human origin.

Applications: ELISA, WB

Recommended Dilutions:

ELISA: 1: 1000- 1: 5000

WB: 1: 500- 1: 1000

Concentration: 1 mg/ ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes E203K mutant, but not wild- type MEK1 of vertebrates.

Storage Conditions: Store at - 20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.