Anti Egfr L747 P753 S Mouse Monoclonal Antibody - USA

Catalog Number: 26287

Gene Symbol: EGFR/ HER1/ ERBB1

Description: Anti- EGFR ( L747- P753) S Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

It activates at least 4 major downstream signaling cascades including the RAS- RAF- MEK- ERK, PI3 kinase- AKT, PLCgamma- PKC and STATs modules.

Defects in EGFR are associated with lung cancer ( LNCR) .

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of ( L747- P753) S, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:

ELISA: 1: 1000- 1: 5000

WB: 1: 500- 1: 1000

IHC: 1: 50- 1: 100

Concentration: 1 mg/ ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR ( L747- P753) S, but not wild type EGFR protein from vertebrates.

Storage Conditions: Store at - 20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.