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  • Anti Pik3ca E542q Mouse Monoclonal Antibody - USA

    price: contact company for price

    Catalog Number: 26210

    Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110- alpha

    Description: Anti- PIK3CA ( E542Q) Mouse Monoclonal Antibody

    Background: Phosphatidyl 3- kinases ( PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival.

    It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit.

    Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.

    Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.

    Applications: ELISA, WB, IHC

    Recommended Dilutions:

    ELISA: 1: 1000- 1: 5000

    WB: 1: 500- 1: 1000

    IHC: 1: 50- 1: 100

    Concentration: 1 mg/ ml

    Host: Mouse

    Format: Liquid

    Clonality: Monoclonal

    Isotype:

    Purity: Purified from ascites

    Storage buffer:

    Preservative: no

    Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

    Species Reactivity: Recognizes E542Q mutant, but not wild- type PIK3CA of vertebrates.

    Storage Conditions: Store at - 20°C.

    Avoid freeze / thaw cycles


    Company Contact:


    • Phone: 6109452007

    • Address: 24 Whitewoods Lane, Malvern , PA , USA

    • Email: Email


    Published date: March 11, 2015

    • Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).

      These unique antibodies do not recognize the inactive (GDP bound) G proteins.

      These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.

      Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.

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