Anti Pik3ca E542q Mouse Monoclonal Antibody - USA

Catalog Number: 26210

Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110- alpha

Description: Anti- PIK3CA ( E542Q) Mouse Monoclonal Antibody

Background: Phosphatidyl 3- kinases ( PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival.

It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit.

Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.

Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:

ELISA: 1: 1000- 1: 5000

WB: 1: 500- 1: 1000

IHC: 1: 50- 1: 100

Concentration: 1 mg/ ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS ( without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes E542Q mutant, but not wild- type PIK3CA of vertebrates.

Storage Conditions: Store at - 20°C.

Avoid freeze / thaw cycles

Company Contact:

• Posted By: NewEast Biosciences

• Phone: 6109452007

• Address: 24 Whitewoods Lane, Malvern , PA , USA

• Email:

• Website: http://www.neweastbio.com

• Business Description: NewEast Biosciences are specialized in generating protein configuration specific monoclonal antibodies that recognize active (GTP bound) G proteins (including Ras superfamily small GTPases and heterotrimeric G proteins).
  
  These unique antibodies do not recognize the inactive (GDP bound) G proteins.
  
  These monoclonal antibodies have been successfully used to immunoprecipitate active G proteins from cell lysates, and for immunohistochemical staining of active G proteins in cultured cells or in tissue sections.
  
  Hence, these antibodies can be used to study the spatial and temporal dynamics of cellular signaling involving big and small G proteins, the future direction of signal transduction studies.